Why multicolinearity in multiple linear regression is not good?

Hi all, I am dealing with data with sevaral features and many of them are highly correlated with each other as well as with dependent variable. In my research on this topics, I found that...

09 August 2020 3,838 5 View

Has anybody experienced an ScFv that binds to an epitope of interest but binding is completely lost when cloned on to an IgG backbone?

While the epitope that I'm working on is a trisaccharide, I guess the same would apply if someone has experienced it working with peptide epitopes. I am wondering if binding specificity can change...

03 December 2019 7,361 3 View

Damping element design for magnetostrictive sensor?

Hi, I am working the magnetostrictive position sensor for level measurement. It would help, if anyone knows what are the design criteria/ parameters for designing damping element for the...

02 December 2019 4,375 3 View

How can I calculate dN/dS within and between species?

27 May 2018 4,083 3 View

Can I used Copper Plate as counter electrode instead of Pt wire for CV/GCD Study in 2M KOH electrolyte for ultracapacitor ?

Many researchers have reported that the size of counter electrode should be larger than size of working electrode. At present I am using 1 cm2 metal oxide coated carbon cloth as working electrode...

04 May 2018 8,495 8 View

What are Various damage models for mild steel i can use in abaqus general static?

i modelling a steel tube in abaqus and i want to investigate its flexural behavior upto the final fracture. The loading rate that i have applied is very slow and earlier i was using j-c model for...

29 January 2018 6,574 3 View

What things to keep in mind while performing backscattered SEM for elemental mapping of titanium alloys?

24 August 2017 2,494 5 View

SVPWM technique for indirect vector control scheme of I.M., How DC link Voltage (supply voltage of inverter) can be decided?

If 3-phase induction motor of 4kw, 400v, 1440rpm is driven by SVPWM technique using indirect vector control scheme, How DC link Voltage (supply voltage of inverter) can be decided

29 May 2016 8,851 6 View

How do I calculate the average Cd?

17 November 2015 7,968 4 View

How can I calculate time average Cd which is not in periodic steady state?

i am simulating flow around bluff bodies and i got Cd v/s Time plot But i am not able to understand how to calculate time average Cd from it because it is not becoming steady state after so much...

27 October 2015 4,257 10 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View