Our podocyte cultures are proliferating with commercial RPMI medium, which contains 11 mM of glucose, and also in differentiating state. However, when we have performed glucose experiments on differentiated podocyte cultures, 5.5 mM is considered “normal glucose concentration”, however should be it considered as “low concentration of glucose”? Should be differentiated podocyte cultures maintained with 11 mM before glucose experiments at 15 mM (moderate) and 30 mM (high)? Any advice would be greatly appreciated.
Thanks
in general 11mM glycose is an increased glucose state. High glucose-conditions were reached at 25-30 mM.
Therefore, you should perform your experiments at 5.5 mM glycose (normal) vs 30 mM (high-glicose conditions) and you should starve cells at least some hours to days in low-glucose medium.
See: Langer et al. J Nephrol. 2016 Dec;29(6):765-773.
You can also cultivate podocytes in low-glucose medium, this would reduce proliferation and survival rate, but would we more...real!