In Micro BCA™ Protein Assay Kit micro plate method, can we heat samples & protein in 60 instead of incubate plate at 37?

11 September 2016 1 3K Report

Our lab modifies the micro plate assay. We heat the eppendorfs contain protein at 60 degree instead of incubate plate at 37.

Aditya Jyoti Basak

BCA assay at higher temperatures could help increase assay sensitivity. You could play around with the time duration of heating and see if there are changes for known standards.

Go through this for a general overview of BCA assays: http://www.gbiosciences.com/PDF/Protocol/Micro_BCA_Protein_Assay.pdf

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