Dears,
Samples were swirled well in the solvent followed by centrifugation at 10,000 g for 10 min. The supernatant was stored on ice for SA measurement. 100 μl of the supernatant was mixed with 0.1% freshly prepared ferric chloride. The volume of the reaction mixture was made up to 3.0 ml and the complex formed between Fe3+ ion and SA, which is violet in colour was determined by spectrophotometry, measuring the absorbance of the complex in the visible region (at 540 nm). :
I want to know:
1- quantity of 0.1% freshly prepared ferric chloride which should be added to the supernatent 2- The type of solution is used to made up the reaction mixture volume 3.0 ml
thanks
I can't answer your question (sorry!), but I will mention that we have had success quantifying SA levels using a relatively simple/easy biosensor assay. A link to the paper is attached for those interested.
https://plantmethods.biomedcentral.com/articles/10.1186/1746-4811-4-28
thanks for your interest Philip Carella
I send an email to Dr. Warreir and he answer about my questions
he use 100 μl of the supernatant was mixed with 2 ml of 0.1% freshly prepared ferric chloride and 900 μl of water.
This process involves the absorption and modification of these heavy metals by plants, and releasing them as volatile s in less toxic forms into the atmosphere for example,Selenium is volatilized by plants as Methyl silinate and makes selenium less toxic
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