The reason is crude plant extract is 1 mg but the phenolic and protein contents are 1056microgram and 1013 microgram. I have a confusion.
No, during quantification, the amount of each component can not be greater than the whole sample used. For example, you cited phenolic as 1056um/mg and protein as 1013um/mg which are equivalent to 1.056mg/mg and 1.013mg/mg. Meaning every 1mg of sample contains 1.056mg of phenolic compound and 1.013 of protein. Not possible. However, the mistake must have been from the equation. If your answer must be in a particular unit, then your starting sample mass must be converted to the unit. That is if your result must be in "ug" then your 1mg must be taken as 1000ug for the purpose of you then calculation. But in must cases people don't bother especially student to handle it without adequate knowledge
Pardon me for the long story, just to make it simple and clear.
Thanks for your answer. I have seen such results in many publications.
Phenolic content and protein content are always vary plant to plant.
It may be possible in those plant which belong to same family.
Agree with you Ajay Sir. But is it possible that 1 mg of alcoholic crude plant extract can contain 1013 microgram of protein?
Dear Ananta Swargiary, you may wish to provide an example of such publication. Mostly samples are weighed in gram. During calculation, the weight is usually taken into consideration. Thanks.
A very critical question. It is impossible taking into consideration that 1 g = 1 x 10^3 mg = 1 x 10 ^6 µg. The question raised here indicates that a component of 1 mg crude sample analysed has a higher concentration than the whole material. Ideally the sub-components of the crude extract should be less than 1000 µg. However, as suggested by Chukwuebuka Egbuna and if it is the case anyway, I will advise that the total weight of the extract e.g 1103 µg/10mg or 1103 µg/1g be used to avoid confusion as raised in this question.
Egbuna sir, I think I shall not give you the details of the Journal and the title of article but you can see the screenshot of the data where I raised my doubt. I quote the line from result "The total phenolic content of the three extracts varied from 867.2 to 1041.8 mg gallic acid/g extract". Plz see the jpeg file.
Same type of results has been reported by many authors. Is it possible or not ?
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