In relation to the Systematic Review and Meta-Analysis of Zhenzhen Pan et Al. (2023) in regards to Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus.
The detection of viral RNA is widely used as the reference standard for the detection of HDV because of its high specificity and sensitivity making it the gold standard. However, what are the possible limitations that can be experienced?
Reference: Pan, Z., Chen, S., Xu, L., Gao, Y., Cao, Y., Fan, Z., Tian, Y., Zhang, X., Duan, Z., & Ren, F. (2023). Diagnostic efficacy of serological antibody detection tests for hepatitis Delta virus: A systematic review and meta-analysis. Viruses, 15(12), 2345. https://doi.org/10.3390/v15122345
Hello, Ms. Cabrera!
Based on the provided material, HDV RNA method of detection have several restrictions. Such that, PCR testing is only capable of detecting HDV RNA when it is being actively replicated. This poses a problem especially during therapy when replication of viral genome is suppressed. The increased proportion and complementarity of the guanine-cytosine content of HDV RNA has also been determined to have caused challenges in amplification. Furthermore, there had actually been no standard PCR detection technique until recently due to the vast genetic variability of HDV RNA. Hence, results from different laboratories are not comparable.
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