How can I select the best plasmid after sequencing?

I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...

06 February 2021 336 3 View

What can be the problem in the isolation of Plasmid?

I've tried transformation of a 1.7 kb gene with DH5 alpha strain of competent cells and also used PuC19 as a control. After selecting colonies and broth-culturing overnight, I isolated plasmid....

27 December 2020 3,596 5 View

How to obtain thermodynamic potential differences of a reaction?

Adsorption reaction on SnO2 surface Say for ex. 2nh3 + 3O- NOx + 3h2o + 3e 2H2S + 15O- 2SO2 + 2h2o + 6e

02 August 2020 216 3 View

In Ag doped SnO2 nanoparticles, can we find silver ions on the surface? In a redox gas sensing using Ag doped SnO2, in what form silver attributes?

In a gas sensing process using Ag doped SnO2 nanoparticles on silicon substrate, in what form silver is present on the surface before adsorption and during adsorption of a target species. Can we...

15 June 2020 9,641 3 View

How can i reduce primer dimers and get my cDNA PCR amplification done?

24 March 2020 1,525 3 View

What is the minimum input concentration & read depth & coverage required for single cell DNA methylome study either by WGBS or RRBS?

11 January 2016 9,324 1 View

Is anyone familiar with the green disposal of both organic and inorganic wastes in bulk, of a Pharmaceutical Industry?

08 April 2015 1,556 3 View

How do we quantitate RNA from a single cell?

For RNA obtained from a single cell not only the low concentration demands a highly sensitive instrument, but also the volume of total RNA isolated is a limitation. Not more that 1-3uL can be...

26 November 2013 1,754 8 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View