In calcium estimation using Alizarin red S assay, how do we arrive at the calcium concentration (in mM) from alizarin red S concentration (in mg/ml)

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SiRNA transfection increased mRNA level...is it possible? or experimental error?

Dear all, I am working on an uncharacterised protein. The function of this protein in mammalian cell is yet to be discovered. I over-expressed the protein did interaction and co-localization...

01 August 2018 9,280 2 View

Problem in adding other molecule in system for molecular dynamics

Hello all, i am working on a protein complex. For a certain analysis, i am trying to add ethylene molecule in system (Protein + water), to check the variation in system. I have inserted 10...

03 July 2017 7,488 3 View

How to define option in g_rms in PBS file

Hello everyone, i am working on gromacs tool (V 4.6.5). Server, in which we work, accepts PBS for job runs. I have prepared my .tpr file on local machine and performed the mdun on server. As my...

16 May 2017 8,272 11 View

Is there any engine with both 2 stroke and 4 strokes. If we design for that what are the factors we should consider?

I want to design a engine with both 4 stroke and 2 stroke for better torque and lower fuel consumption. I want to know if we design a engine with both 2 stroke and 4 stroke, what would be...

09 April 2017 4,955 9 View

I am working on DNA-protein complex MD in gromacs, how ever stuck on a step where in grompp step getting 'Fatal error: No such moleculetype DNA' ?

I am interested in DNA-protein MD. I have a custom made structure of a DNA motif, i have edited it (from ATGC to DA, DT, DG, DC) for gromacs and generated topology using amber99sb-ildn.ff. After...

12 February 2017 4,599 4 View

Why the shifting in absorption peak when gold nanoparticle modified with mercapto acids?

Not available

05 May 2016 2,460 1 View

Why am I not getting structures in synthesizing of bimetallic core-nanoshell nanoparticles?

I need some help synthesizing bimetallic core-nanoshell nanoparticle of Ru:Pd:Ni. Why am I not getting such structures? Can anybody help me out?

05 May 2014 2,699 1 View

Can someone shed some light on whether my protein forms inclusion body?

1. Recently we did transfection of our gene of interest (cloned in pEGFP-C1 vector) in HeLa cell, and we found that only few cells were transfected and those few cells show a diffuse localization...

11 December 2013 2,048 4 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View