Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

What is the best solution for counter electrode for perovskite solar cells at room conditions?

I'm investigating transparent counter electrode for FTO/PEDOT:PSS/MAPI3/PCBM layered perovskite solar cell. In my research lab, currently i have a glove box with N2 gas as a carrier. I'm goning...

21 December 2020 6,504 3 View

My status has changed from "Awaiting Reviewer Score" to Awaiting AE Decision". Could the Associate Editor reject the manuscript at this stage?

Otherwise what this status means. If the Editor has received the comments from reviewer then why he is not sending it to Authors. As 10 days already spent till the status changed.??

18 November 2020 6,252 4 View

How to adapt a mature Questionnaire?

I am looking forward to seek some help regarding adaptation of mature questionnaires. I am new to quantitative studies and wants to use some demographic in my questionnaire, is it necessary to add...

08 November 2020 7,146 5 View

Is there a way to isolate nuclei without purifying cytoplasmic protein aggregates?

Hello, I am trying to isolate nuclei from HEK293T cells expressing a protein that aggregates in the cytoplasm, however when I run my samples in western blot the cytoplasmic protein is strongly...

02 November 2020 9,541 3 View

How can i fill zinc oxide or titanium oxide into etched pattern on glass substrate?

Hi! I need zinc oxide or titanium oxide to be filled into etched area on glass sbstrate. Etched are is being photolithography processed and being dry etched. Material must not present around the...

25 October 2020 152 7 View

How to get the most out of your qPCR data?

23 October 2020 7,537 3 View

Do you think that the e-learning that took place after the COVID 19 crisis was fair for students to evaluate their knowledge?

The significance of the question is to know the extent of accuracy in evaluating students.

19 September 2020 3,663 13 View

How to calculate reliability for a newly launched system?

We have a hydraulic system (water collector and distributor) which is newly started, how can we calculate its reliability?

07 September 2020 6,619 2 View

Neonatal Renal dose adjustment! Guidelines or the reference that is most authentic in clinical practice.??

In the infants to the adult age group, we have been given a concrete guideline for dose adjustment on the basis of CKD-Stages or creatinine clearance. this pathway is missing in neonates but...

07 September 2020 1,731 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

What will happen when we assume an upper bound of OFV lower than the global minimum OFV ?

if we assume 20 is the upper bound of an objective function (OF) based on permutation a square matrix (nxn) , The minimum value of the OF=22, then we run a random operator to compute the OF by...

14 February 2021 6,056 5 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View

Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View