In a case of coinfection with salmonella and Ecoli in pigs, which one is responsable for the immundepression?

More Mariem Botrus's questions See All

I need detailed steps of pretreatment of macroporous Resin Amberlite XAD 16N ?

No informations furnished from the supplier. I am working with this type of resin for the first time! In litterature, I found these steps: - Use deionised water to remove salts until neutral pH...

12 March 2024 4,612 0 View

Which cell lysis buffer to use for a pull down assay?

Dear all, I have a bacterial protein that's internalized in human cancer gastric cell (AGS cells). I want to search for its partner so I am planning for a pull down assay. Which is the best cell...

31 October 2022 1,052 3 View

Material orientation coordinate system in Abaqus?

Hello, I’m working in the modeling of the wood peeling process. The Hill criterion is used to model the anisotropic material behavior. In addition, the material orientation is considered through...

05 September 2022 1,344 0 View

How to plot the campbell diagram with all the harmonic orders?

Hello, I'm using ANSYS for computing the campbell diagram but i cannot plot the harmonic orders in the same graph, i can only plot 1P or 2P.. but not in the same graph. is there any one know how...

29 August 2022 229 0 View

Where are unmasked faces in RWMFD dataset?

Hello, I have download the RWMFD dataset. But I can't find the unmasked faces of subjects. In the description of the dataset they mentioned that: The dataset is further organized into 7178...

19 May 2021 1,565 6 View

How to remove fatty acid from protein?

Hi everyone! I have a protein that I suspect that binds fatty acid. I want to get the free form of the protein (without its ligand). So i will use the Hydroxyalkoxypropyl-Dextran Type IX...

26 April 2021 2,517 4 View

Bismuth chloride solubility?

Hi everyone ! is it possible to solubilize Bismuth chloride in ammonia solution. If not, what is the maximum solubility of this compound in water. thank you. Mariem

14 June 2020 3,485 4 View

How to analyze result obtained from ddPCR using cDNA as template?

hello, I did ddPCR for my cDNA and I obtained the result on number of copies per micro-liter. how to normalize it compared to the [total RNA] ug/ul?

13 January 2020 3,151 2 View

How to calculate the optimal resistance, for the case of energy harvesting of the piezoelectric cantilever?

For the case of energy harvesting of the piezoelectric cantilever , the electrical power output delivered to the resistive load is measured by the equation : P=V2 / R. Where: R: the...

27 April 2019 6,196 5 View

How can I compute the photometric transformations between two images?

Hello, Given two images of the same scene, I need to know the photometric transformations between these two images. These images are taken by cameras embedded on a robot. Thank you,

22 December 2015 8,290 4 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,605 2 View

Best method for extracting RNA from morulae?

Best method and number of embryos for extracting RNA from pig morulae?

21 July 2024 4,398 1 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

Need a reference on pig residual analysis report and the parameters containing in it, for an oil and gas pipeline industry. Has anybody done it?

Pigging is basically a cleansing process followed in all pipeline industries after the product has been delivered. There are many types of pigs that are inserted inside the pipeline for this...

27 June 2024 1,221 1 View

How to add one of my recently published papers that has not been uploaded in the ResearchGate?

To whom it may concerns, One of my recently published papers has not been uploaded in the ResearchGate. Physiological Basis for Xenotransplantation from Genetically-Modified Pigs to Humans: A...

28 May 2024 8,172 2 View

Assistant with TUNEL technique for A549 cells, switch from trypsinized cells to adherent cells?

Hi, Im using the kit from abcam (ab66108) In situ Direct DNA Fragmentation (TUNEL) Assay Kit for A549 cells, the kit required to trypsinize the cells which its okay (in theory) but the problem is...

07 May 2024 4,706 0 View

What are local means to reduce pig death by natural diseases?

my pigs are dying what can i do please help researchers

07 May 2024 993 9 View

What are Trends in viral infection among cancer patients?

Hello every one Can any one suggest me trends subjects in viral infection in cancer patients for PHD thesis? Thanks in advance.

05 May 2024 5,263 3 View

What is the breakpoint diameter for florfenicol and oxytetracycline in the disk diffusion?

What is the breakpoint diameter for florfenicol and oxytetracycline in the disk diffusion method, indicating susceptibility or resistance to these antibiotics in the tested salmonella strain?

25 April 2024 5,168 1 View

Please has anyone used Enrofloxacin with disc content of 10, Vancomycin (30) and Cefamezin (30) for disc diffusion method? What was the breakpoint?

I used these antibiotics, such as Enrofloxacin (10), Vancomycin (30), and Cefamezin (30), for Salmonella spp. as a disc diffusion method, and I'm finding it difficult to get the breakpoint for...

04 April 2024 2,710 0 View

Brian Thomas Foley

There are thousands of species and strains of Salmonella and millions of strains of E. coli. Some of each can be highly pathogenic, and many more are slightly pathogenic in pigs. I don't think there is any single correct answer to your question.

Mariem Botrus

thanks alot for your answer, in a case of the detection of salmonella in intestine after anreichung in the cell culture, can it causes several changes in the intestine?

I am not an expert on pathogenesis of Salmonella, but a PubMed search brings up dozens of papers:

Article SipA activation of caspase-3 is a decisive mediator of host ...

Article Host Restriction of Salmonella enterica Serotype Typhi Is No...

Article PERP, a Host Tetraspanning Membrane Protein, is Required for...

etc...