Hi all,
Generally, what sample mass (proteins) is required to perform glycosylation profile analyzes of recombinant proteins?
What to do when you have a small sample?
What are the main strategies used?
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
Please inform me about the International Conference on Plant Molecular Biology, or Plant Biology, scheduled for December 2024, January/February, or March 2025.
14 July 2024 3,120 7 View
need to know protocol for photoplate creation, styles, etc
11 July 2024 8,321 2 View
How can anyone develop a theoretical framework that successfully unifies general relativity and quantum mechanics? how this new theory should accurately describe gravitational interactions at...
10 July 2024 5,040 8 View
I am able to submit the code documents. Although my graph is 1, I would like to be in the 6E-05 graph range. Please, someone correct the error.
08 July 2024 5,770 0 View
I have tried to optimize the serum free dmem which is the best but i am afraid the secretory profile has too much variations.
03 July 2024 4,044 3 View
Please help me with one or more of the following, if possible: How does a client mentally process the trauma? What are the cognitive domains of processing? What measures are available to assess...
02 July 2024 6,263 0 View
The NS1 protein of the tick-borne encephalitis virus potentially has three glycosylation sites. When processing the PNGase F protein on SDS-PAGE, I observe three bands - a fully glycosylated 52...
01 August 2024 3,519 4 View
I have conducted a survey on the "effect of vacuum drying conditions on carbohydrate content of angelica.S". The result show that, when increasing the vacuum degree, the carbohydrate content first...
13 July 2024 592 1 View
The line appears always in the penultimate run. We try to decrease the volume of the solvent system, wich is composed of butanol:acetic acid:water (2:1:1) (76 mL) but the line remains. Below I...
24 June 2024 9,829 3 View
Previously I have used GlyProt to add carbohydrate chains with my modelled protein, but the server is not working now. Is there any alternative tool for modeling glycosylated protein?
01 June 2024 894 1 View
Hello esteemed researchers, I am seeking guidance on how to calculate the total carbohydrate content. I've observed that some researchers divide the concentration found by 0.1 and then multiply it...
14 April 2024 8,888 8 View
Carbohydrates are still considered a non-essential nutrient. But if we include dietary fiber, except in specific adaptive contexts of particular ecological niches, is this a reality today?
11 April 2024 6,566 0 View
in optimization process its showing for carbohydrates computational studies. How to Solve Inaccurate quadrature in CalDSu. errors in Gaussian?
05 April 2024 1,389 1 View
Hello I need suggestions, As I wanted to estimate sugars from bacteria(pellets/biomass) sample by phenol-sulfuric assay but not sure how to extract carbohydrates from the bacteria source. Thanks
16 February 2024 6,914 1 View
Mucilage has been isolated from a plant material by extraction with sodium carbonate solution and precipitating the same by change of pH to acidic condition. What tests need to be done to...
14 February 2024 8,053 1 View
Hello, I want to use a commercial preparation of recombinant PNGase, but I do not have the buffer. What cofactors or buffer conditions does PNGase F require? I will add the enzyme to cell...
08 February 2024 5,715 1 View