In a Caco-2 permeability assay can I use HBSS with DMSO for my test compound and plain HBSS for the permeability standards? Or do I have to use same?

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Thomas J Raub

You should always match the final conc. of DMSO.

Napoleon Nikolaos Vrettos

Thank you for your reply Thomas. Yes your are right for sure, but is it ok for me to use HBSS with DMSO for the test compound and plain HBSS for my permeability standards (i.e without DMSO)? Or does it have to be the same medium for all of them?

Janice Goh

Same medium is important for all just in case DMSO does anything to the cells