Hello,

I'm having trouble getting well-resolved SDS-PAGE gels after elution from streptavidin beads. My biotinylated well looks mostly like one long streak, with very little resolution of any bands. See the attached image. For reference, I'm trying to do proximity labeling with a riboflavin photocatalyst. Lysates are enriched with 40 uL of elution buffer: 375 mM Tris-HCl (pH 6.8), 9% SDS, 50% glycerol, 6% β-mercaptoethanol, 20 mM DTT and 2 mM biotin. I typically run half of the sample on gel (20uL). Has anyone had similar issues or know how to fix this?

Thanks