Hello,
does anybody have recommendations for staining CD122 (IL-2Rb) on T cells for FACs?
I tried staining it for 30min at 4°C (SF staining) on cultured CD4 T cells using an aCD122 antibody from biolegend, but the signal barely differed from my FMO.
Could it help to starve the cells for IL-2 a couple of hours prior to the staining?
Would appreciate any input!
Thanks,
Valerie