Hi. I activated qiime 2 2019, prepared a folder named '6 sample file' to try data import which has metadata, manifest, and 6 data sequences as in the picture. I received this error. maybe as manifest and metadata are not qza (I read the tutorial but could not find how to convert into qza. Thanks a lot
(1/1?) no such option: -q
(qiime2-2019.7) a-PC:~ hebahussein$
(qiime2-2019.7) a-PC:~ hebahussein$ qiime tools import \
> --type 'SampleData[PairedEndSequencesWithQuality]' \
> --input-path 6 sample import \
> --output-path paired-end-demux.qza \
> --input-format PairedEndFastqManifestPhred64V2
Hi Heba Huessin ,
You don't have to convert the files into qza, QIIME can identify fastq files.
The image you provide shows that your fastq sequences are in zipped folder. unzip the file before proceeding further.
One more thing, you can not use metadata in yml format for QIIME analysis. You need to create a valid "mapping file" in QIIME's preferred txt format.
Its worth mentioning that spaces are not recommended when you work on command line, if you have to name something add _
Try to put a screenshot of your console so its easy to see,
As Shan say, you dont convert into .qza or qzv... these are output extensions of Qiime2.
(1/1?) no such option: -q
This is maybe because you put on the option values instead of a filepath:
> --input-path 6 sample import \
This should be a directory, like this:
/Your_name/Desktop/6 sample import
Its worth mentioning that spaces are not recomended when you work on command line, if you have to name something add _
> --input-path /6_sample_import \
Also manifest file should be a Tab Separated Values extension, or .tsv
Hope this helps,
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