I am working on HT29 cholorectal cancer cells. I have started an immunofluorescence staining protocol on them. I have previously fixed the cells with 2 % formalin diluted from the original 10 % formalin.
For this I added:
1 ml of the 10% formalin with 5ml of PBS.
Now I am going to fix the cells with Paraformaldehyde. What I have is 4% PFA. So I am wondering do I need to dilute the PFA to 2 % as well? And if so how much PFA and pbs needs to be added?
thank you in advance.