Do anyone have come across write up on "Technology on De-biasing" ?

De-biasing is a term applied in psychology, behavior, and management decision process. Now it has some connection with technology. Technology is used for leveraging on the reduction of impacts of...

21 January 2021 4,572 2 View

Can anyone help me the protocol/techniques to detect the concentration of dyes (Toxic chemicals) in tissues?

I want to detect the concentration of toxic dyes (Toxic chemicals like different pesticides, herbicides etc.) in certain fish tissues. Therefore, I need protocol or techniques so that I can detect...

06 September 2018 2,168 3 View

What could have gone wrong in the 2Dgel where the protein (soluble fraction) seem to have aggregated at low and high pH?

I am new to 2D gel electrophoresis. I tried to follow the basics but the results have come as an absolute shocker. As a newbie my troubleshooting skill is as limited as my hands on skill in the...

20 July 2018 5,140 4 View

Perovskites are a class of compounda, than how can we identify that which material is suited for harvesting solar energy?

26 February 2017 4,620 3 View

How do I calculate pore water saturation of concrete

Suppose I have concrete of M20 grade, volume is 150 cu mm. Now, how do I calculate the pore water saturation?

08 May 2016 459 3 View

What are the thicknesses of each of metal layer 1 to metal layer 6 in 180 nm CMOS Ip6M?

What are the thicknesses of each of metal layer 1 to metal layer 6 in 180 nm CMOS Ip6M? Also what are the thicknesses of each of the oxide layer separating them and the silicon layer at the end?...

09 April 2016 4,727 3 View

I am getting a PCR amplification from a brain region, albeit a faint signal, while in RNA in situ I am not. Is this normal?

The signal varies with physiological state and RNA in situ signals are present when the transcription is quite high. The probes are all working and the protocol is fully standardized Is it so that...

21 October 2014 3,602 3 View

How can I test the production of mycotoxins by mould isolates?

03 September 2014 4,262 8 View

What might be my problem with agarose gel electrophoresis?

07 July 2014 7,989 20 View

What is the effect of polarized and nonpolarized electrodes in the case of polarography? How is the concentration gradient formed in the solution?

How is the diffusion current maintained at the limiting plateau? If the diffusion current is formed because of the discharge of charges for reducible ions, then how is the diffusion current...

09 December 2012 1,892 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View