Do anyone have come across write up on "Technology on De-biasing" ?

De-biasing is a term applied in psychology, behavior, and management decision process. Now it has some connection with technology. Technology is used for leveraging on the reduction of impacts of...

21 January 2021 4,572 2 View

Why is there cell debris are coming after 2nd or 3rd passage of the cells???

Our lab is facing a difficult problem for almost a year. After thawing the cells are okay, but after 2nd or 3rd passage there are debris present between the cells. We use mycoplasma removed cells...

17 January 2021 5,629 6 View

Can you help me for generating chicken swarm optimization matlab code?

Multi-objective optimization through chicken swarm optimization technique. Both maximization and minimization problem.

31 December 2020 2,299 4 View

Any good methods for double-stained markers quantifications from tissue sample using computer programs?

How can we best quantify double-stained markers when comparing diseased and control? For example, if we want to stain one marker of smooth muscle cells and compare the expression in diseased and...

21 December 2020 7,389 1 View

Membrane protein Expression Purification: Broad Asymmetric peak on size exclusion ?

Hello, I am expressing an integral membrane protein (MW 51kDa) in Hi5 insect cells. I initially purified the protein by Nickel-His, concentrated it and subjected it to Size Exclusion...

10 December 2020 2,781 5 View

How should I store the milk sample in RNAlater?

I want to extract RNA from human breast milk. How should I store the milk sample in RNAlater? And after RNA extraction how can I store the RNA?

01 December 2020 7,183 4 View

Can U suggest standard protocols of quantifying total phenolics & total flavonoids using gallic or tannic acid for the former and quercetin for later?

The problem is that everytime getting the flavonoids content much higher than phenolics which may be, but in rare occasion. In phenolics, I am adding100 ul sample with 900 ul dH2O with...

29 September 2020 4,993 6 View

What is the ideal Elution buffer composition for a His tag protein purification to prevent protein aggregation after freeze thaw ?

Hi All, Recently, I purified 8 proteins and stored at -20 with the elution buffer containing 20 mM Tris, 250 mM NaCl and 200 mM Imidazole. I autoclaved the buffer, so I do not know whether the...

25 September 2020 9,814 6 View

What is the use of having multi-wavelength absorption measurements in Aethalometer?

Aethalometers are used to estimate the light-absorbing aerosols or Black Carbon (BC) or Soot particles. There are widely used aethalometers which are measuring light absorption in multiple...

06 September 2020 1,050 3 View

Has anyone done magnetic target localization using Genetic Algorithm?

I am trying to model magnetic target localization in MATLAB using those algorithms. So essentially what I want to output is the position vector of the target (x, y, z) and the magnetic moment...

28 August 2020 9,811 2 View

How to fix the voids on a floodplain map by using HEC_RAS model?

24 January 2021 9,054 2 View

How the precipitation Ratio and flow Ratio effect the output of the result?

I am doing HEC HMS calibration than i want to increase simulated value and volume of the computed so that my issue is how the precipitation and flow ratio make influential that result?

22 January 2021 152 2 View

Can anyone please tell me, what will be the Crop Coefficient value for shortleaf pine and oak tree in HEC-HMS model simulation?

my study area located in the forest region having Shortleaf Pine and Oaktree, thus in the Canopy Method I am trying to get the Crop Coefficient value of these trees but did not get anything.

18 January 2021 9,789 1 View

HEC RAS: project file error?

When I try to create a new project file on HEC RAS, this message appears "title and file name must be specified", even when I have already specified them.

03 January 2021 5,618 2 View

How to download future precipitation data for years 2021-2030?

For my future discharge forecast in the calibrated HEC HMS model, i need the precipitation data of the future.How can i get this data in the daily timescale?

21 December 2020 8,928 5 View

How to perform snowmelt runoff modeling in hEC HMS?

My study area of rainfall-runoff modelling is in mountainous region and has snow cover. There is about 10 percent snowmelt contribution to the discharge of the river i am modelling. How can I...

23 November 2020 7,873 4 View

How to solve the longest flow path error in HEC-geo HMS?

I'm having issues running the Longest Flow Path tool in HEC geo HMS. Attempting to run the Longest Flow Path tool results in the following warning error message. The error is attached. All...

19 August 2020 9,365 4 View

How can i classify these soils obtained from SOTER database to different Hydrological soil groups reguired in HEC HMS CN method??

I dont have any other soil maps for my study area.. How can i use this data to convert it into Hydrological soil groups A,B,C or D ?

04 August 2020 7,848 6 View

How do I determine the correct ratio to peak value for the Recession baseflow in HEC-HMS?

I am wondering if there is a way to estimate the ratio to peak value without calibration? What would be the most suitable value if I don't have a measured hydrograph to compare? Thanks for any...

27 July 2020 2,242 1 View

How do I estimate initial baseflow for each subbasin (HEC-HMS) ?

I have one hydrograph close to the watershed outlet from which I can extract a baseflow value. But what values would I assign to all the other subbasins? The HEC-HMS user manual says the...

21 July 2020 5,431 3 View