im working with lipase produsing bacteria, i am faceing a problem with isolatin media and i am not getting clear zones , is any body cen help me ?
im using Tributarn agar,tween20,80,and RhodomineB agar, but im not getting any clear zones
You did not mention the details of your assay media. You might want to check out the following thread.
https://www.researchgate.net/post/How_to_test_lipase_activity_of_microorganisms_on_a_petri_dish
You did not mention the type of samples , try with these details
Lipase/esterase producing micro-organisms produced a zone of clearance (hydrolysis) when their appropriate
dilutions were spread on the TBA medium containing per liter of peptone, 5g; beef extract, 3g; tributyrin, 10ml and
agar-agar, 20g. The zone size was measured after 24 and 48 h of incubation at 37°C.
Dear Sir.,
I use procedure in this paper, its easy and the oil common in your lab
good luck
Hello Nagaraju,
Bacterial lipases are greatly influenced by nutritional and other factors, such as temperature, pH, nitrogen and carbon sources, inorganic salts, agitation and dissolved oxygen concentration. So, these factors to be taken care while testing any bacteria for lipase activity.
The ability of organisms to hydrolyze fat is accomplished with the enzyme lipase so, this test was conducted to study the ability of PHAs producing bacterial isolates to hydrolyze lipid by the action of enzyme lipase. Thus, tween NA plates were prepared reducing the concentration of nutrient broth to five times. Then 1% [V/V] tween-20 and 1.5% agar was added to the culture medium as lipid and solidifying agent respectively. The isolates were spot inoculated on the plates and incubated at 37oC for 24 hours. Moreover, plates were then stored at 4oC for 2-3 hours to precipitate the lipid in presence of CaCl2. A clear zone around indicated positive lipolytic activity of the isolate caused by the production of lipase.
Dear Nagaraju
I want primer responsible for lipase in G-ve bacteria plz
if u can help me
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