Specific details on motor specs would be appreciated. Is there a specific rpm or torque required? Or will any 12V motor with an appropriate shaft size do the trick?
Recommended tape adhesives that work well with PCR would also be helpful. Paraffin/hexane is one possibility from the lit.
As far as the recommended adhesive, you can buy "plate seals" for 96-well PCR plates. Make sure they are rated for PCR and not just general storage. Cut them into any size strips that you would need to cover part or all of a 96-well plate.
Thanks Katie. I was actually asking about the adhesive used to trap the spores inside the Burhard spore trap. Was that your intention?
Ah, I thought you were looking for something to cover the tops of the plates during PCR.
You might want to look into DNA extraction kits for paraffin-embedded samples and/or high oil content samples. Lipids can really inhibit DNA extraction and pollen grains are fungal spores are already challenging due their hard coating.
I'd suggest freezing & grinding in liquid nitrogen to disrupt the coating. Maybe an "old school" phenol-chloroform DNA extraction protocol to eliminate more of the lipids.
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