Why the battery connected on the load side for storing purpose is taking the volatge /current at whatever rating it is given in matlab simulation?

I am designing a DAB DC DC Converter and when I am connecting a battery block in Matlab and fix its specification lets say 350 V and 25 A, when I am giving input as 600V, Battery would charge to...

11 February 2021 3,110 1 View

How do I connect DAB DC DC Converter to a battery in matlab simulation ?

I am trying to model a DAB converter and I need to observe the power transfeered to the load side on varying the phase shift between two bridges, hence I couldnt take a constant load e.g...

09 February 2021 6,489 3 View

After G actin polymerization and labelling I didn't got filaments. Why?

Purified G actin and got 42kda band and an additional band just below it. While performing G actin polymerization and labeling with rhodamine-phalloidin I didn't observe filaments under the...

20 January 2021 3,751 2 View

Why there is need to calculate the chlorophyll content and chlorophyll index. What protocol should I used for calculating chlorphyll in mustard?

I want to know the best and effective method/ protocol to calculate the chlorophyll from mustard plants. Please suggest some relevant and the methods or protocols from which I can easily calculate...

05 January 2021 9,882 3 View

Only one clear RNA band on gel?

The RNA quantity in nanodrop is about 1 microgm. 260/280 ratio is also around 2, still I am not getting clear bands on gel. What can be the reason?

30 December 2020 1,731 2 View

How can we calculate the stoichiometric ratio of MoOx from UV-Vis diffuse reflectance/absorption spectra?

I have read one research article by M Dieterle et al. In this article they have discussed about finding stoichiometry of MoO3-x using DR UV-Vis spectra. I am not getting the required equation they...

18 December 2020 9,755 2 View

How to plot Taylor Diagrams?

Hello, I need to plot Taylor diagrams for some of my analysis. However, I am not sure how can I plot them either in excel or Matlab. I have searched on several sites, I could not reach any final...

17 December 2020 5,228 3 View

Bacteria growing well on nutrient broth but not on NA plates. What could be reason? How can it be resolved?

I am facing a problem. I have isolated few bacteria from soil and preserved them in glycerol stock (50 % glycerol v/v). Now when I am inoculating these cultures in nutrient broth and incubating...

06 December 2020 3,577 6 View

Is calcination necessary for synthesis of Pd deposited CeO2 nanomaterials?

I want to synthesize Pd deposited CeO2 mixed metal oxide composites

03 December 2020 3,195 6 View

Can anyone kindly explain me about the chirality of DNA?

As DNA is a macromolecule made up of sugar, phosphates and bases than how can we consider it as a molecule? And secondly which type of experiments we can run to test for the chirality of it? There...

28 October 2020 8,583 3 View

Which tool for operon prediction in bacterial genomes ?

Hi, I want to predict operons on entire bacterial genomes (already annotated). I used to use operon-mapper ( https://academic.oup.com/bioinformatics/article/34/23/4118/5040321), which is great but...

07 October 2020 7,192 7 View

What would be the likelihood of different environmental trigger for the same virulence operon across isolates?

So, I am looking at condition regulating the expression of a group of virulence gene. I have several genes, all supposedly on the same operon, and, sure enough, they all seem to react together...

25 August 2020 1,753 3 View

How to make operon construct for homologous recombination mediated gene integration in E. coli chromosome?

I am planning an experiment where I will integrate an operon construct in the gfc locus E. coli using CRISPR-cas9 mediated homologous recombination. To insert my operon construct in the gfc locus,...

04 May 2019 9,163 3 View

Chapter invitation from springer book 'Functional Operons in Secondary Metabolic Pathways of Fungal Origins Vol I and II'?

08 September 2018 9,089 2 View

Which is the best tool for operon prediction in bacteria?

I'm looking at the gene sequences of a pair of enzymes and related processing proteins and am trying to figure out whether they are in the same operon, and where the operon begins and ends.

08 September 2018 8,459 8 View

Protein based inducers for gene expression?

Hello all, I'm looking for amino acid, peptide or protein based inducers in gene expression and the repressors they bind to, also is there any database available for this?

04 May 2018 8,893 0 View

Drug inhibits protein, but the specific gene also get no or lower expression after 24hrs of incubation ?

An enzymatic functional protein has been targeted using small molecule compound. We succesfuly found that the compound has inhibited the growth of the organism. But, we found mRNA for that...

03 April 2018 818 4 View

Problems with promoter fusion with lux genes?

I have been constructing some promoter fusions using the lux operon as a reporter in a miniTn7 transposon, which is inserted in a neutral region in the genome. Everything is fine until I...

10 November 2017 4,369 2 View

Fifty Years after Jacob and Monod: What Are the Unanswered Questions in Molecular Biology?

09 October 2017 8,757 22 View

How to identify genus and species of a bacteria through 16S rRNA gene sequencing that showing only 90% sequence similarity with reference strain?

Hi... I have done 16 S rRNA sequencing of some of my bacterial isolates and one of my isolates is showing only 90% sequence similarity with reference strain as the maximum identity. Should I...

03 April 2017 2,394 6 View