Getting the highest band at 3.5kb but require the whole 5.1 kb operon for overexpression
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...
25 July 2024 9,229 1 View
Is it necessary to add stop solution in Elisa plate?
30 June 2024 8,235 2 View
Hello! I am facing a problem, I tried using pdb2gmx to generate topology file but it shows error HEA residue not found then I tried copying the forcefield file and edited accordingly but again...
24 June 2024 1,242 0 View
We are willing to make notice of Miller indices present in XRD powder and managed as their JCPDS files. We need them for various substances, e.g. Graphite, copper, lithium, and Iron. Kindly share...
23 June 2024 864 2 View
After Plagiarism checking in Turnitin, the result shows that the similarity of my manuscript is from ''Article with reviewer- feedback from Springernature'' and the link provided opens a word file...
20 June 2024 8,325 1 View
I have tried to screen various phytochemicals of different plants leaves sample with antimicrobial property. I have tried aqueous extract of plants. But i have doubt whether sample is actually...
18 June 2024 8,947 4 View
I am planning to do invitro transcription and capping for an mRNA vaccine generation. Can anyone suggest whether it should be done on a bench top or inside a laminar hood?
14 May 2024 186 3 View
How can we use Ovito to do this. I have deformation behavior results from MD simulation under uniaxial tension and I want to see misorientation .
01 May 2024 7,211 0 View
Restriction enzyme that i used for double digestion is EcoR1 and Xho1 for 1,2 and 3 hours but didnt get digested band on gel. RE is of Genei company.
01 May 2024 3,580 1 View
Hi everyone, I was looking for some tools (preferably web-based tools) to predict genes organized in operons in various bacterial sequences I currently work on (plasmids, chromosoms, etc.). For...
19 February 2024 8,922 2 View
Has anyone ever observed a percent identity of the order of 97% between two operons of the intergenic region 16S-23S rRNA in a same cell ? Do you have some references ?
08 January 2023 7,882 3 View
Does anyone know whether the tracrRNA, Cas9 and the CRISPR locus in the CRISPR-Cas9 system belone to the same operon? Thanks in advance!
10 August 2022 9,243 0 View
I'm trying to purify a natively expressed protein from a large operon, native expression levels are very low, does anyone have any experience with changing the innate promoter with let's say an...
04 June 2021 2,301 4 View
Target bacterial operon is reported to be co-transcribed and co-translated in wild type host. However, protein expression analysis (SDS PAGE) indicates translation of only first gene in frame...
15 April 2021 3,921 3 View
Can someone help with any tips on how to look for the gene islands, what software to use for alignments, and build the tree. I have already constructed using only 1 primary gene in the island and...
15 February 2021 8,600 3 View
Hi, I want to predict operons on entire bacterial genomes (already annotated). I used to use operon-mapper ( https://academic.oup.com/bioinformatics/article/34/23/4118/5040321), which is great but...
07 October 2020 7,588 7 View
So, I am looking at condition regulating the expression of a group of virulence gene. I have several genes, all supposedly on the same operon, and, sure enough, they all seem to react together...
25 August 2020 1,938 3 View
I am planning an experiment where I will integrate an operon construct in the gfc locus E. coli using CRISPR-cas9 mediated homologous recombination. To insert my operon construct in the gfc locus,...
04 May 2019 9,316 3 View
Dear Drs., We cordially invite you to contribute a manuscript in our proposed books on the major theme ‘Functional Operons in Secondary Metabolic Pathways of Fungal Origins Vol I-Fungi as Foes’...
17 September 2018 9,756 2 View