What's the difference between 0.1% triton-X100 and ice-cold methanol in the immunofluorescence assay for intracellular protein staining?

Hi, Guys, I noticed that researchers usually permeabilized the cells with 0.1% Triton-X100 or ice-cold methanol (90%) for an intracellular protein staining, it looks like that the two methods work...

15 February 2021 5,606 4 View

How to effectively limit the output range of the intermediate layer of the cascaded neural network used in the reverse design of metamaterial?

The use of cascaded neural networks for the reverse design of metamaterials and nanophotons can effectively alleviate the problems caused by one-to-many mapping, but the intermediate layer of the...

05 August 2020 2,612 1 View

Is the existing Quantum Computer able to break the current 2020 DLT/Blockchain encryption ?

According to Google, it can achieve a quantum dominance with 53 qubits, i.e. 253 calculation simultaneously. However, this is not sufficient to break SHA2562 encryption through brute force with...

31 July 2020 2,424 3 View

Sticky black, irregular patches in cell culture flask?

Hi all, Recently I encountered some irregular black/grey patches inside my cell culture flask (see attached image, x400 magnification). They are not motile, not floating, do not stick to cells...

08 July 2020 7,921 3 View

Transfected cells died after trypsinization ?

Hi all, I am facing a problem to trypsinize and combine/move the transfected cells into new well . I was planning to pool the transfected cells into a new well to ensure better cell-cell...

21 December 2019 9,692 5 View

Problem with stable cell line generation?

Hi all, I am facing a problem to generate a stable cell line after successful transfection. I added puromycin to kill the untransfected cells, however, eventually all my transfected cells are...

19 September 2019 2,970 2 View

How to convert the arraystar circRNA number to circbase?

Several studies only showed the circRNA name from arrarystar, eg.mmu_circRNA_22066, without providing the spliced sequence or its position on chromosome. It will be hard for others to follow their...

20 August 2019 2,206 2 View

Co-transfection of miRNA mimics and inhibitors?

Hi, Is there any paper which reported the co-transfection of miRNA mimics and inhibitors? Thank you.

17 June 2019 1,087 3 View

MiRNA primer sequence?

Dear all, I have a miRNA primer sequence left by someone else and I would like to know about the sequence of this primer. Is there any method I can use to sequence and know the sequence of this...

14 June 2019 5,304 6 View

Qiagen or ThermoFisher Lipofectamine Transfection Reagent?

Hi all, I am planning for some transfection work involving a mammalian cancer cell line. Can I know the transfection kit from Qiagen or ThermoFisher lipofectamine is a better option? Do you...

23 March 2019 9,831 3 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View

Does anyone knows about a really easy but still comprehensive ImageJ tutorial?

I am new to this software. I am in need of analyzing images from ocular ultrasounds (black/white) and find a way to quantify the internal reflectivities from the vitreous cavity in cases of...

16 February 2021 4,238 2 View

How to measure fluorescence intensity of Dapi stained nuclei?

I have tried various methods using imageJ to measure mean fluorescence intensity of dapi stained nuclei but none of them seem to be working. Ive attached an image i am trying to measure the...

15 February 2021 8,637 3 View

Benefit of CTCF (corrected total cell fluorescence)?

03 February 2021 6,302 2 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

How can I analyze the morphology of microglia (ramification, braches, soma, etc.) via Fiji/ImageJ?

Hey everybody, for my project I need to analyze the morphology of microglia, which I have previously stained (IHC) and scanned in a SlideScanner. Specifically, I need the ramification, number and...

31 January 2021 3,855 3 View

How credible is the average size of nanoparticles obtained using SEM image and ImageJ software?

My research work requires synthesizing nanoparticles, characterisation before application. How credible is the average size obtained from ImageJ software using images from SEM or TEM?

30 January 2021 3,838 3 View

Which software to use to statistically analyze Telomere Length_TRF_Southern Blot?

I am trying to measure telomere length in an ant species using the TRF method, which is a Southern blot technique. Currently, I am struggling with analyzing the images and would appreciate any...

29 January 2021 604 3 View