I'm new to HER and ORR reactions and have been tasked to find why we set the RDE to 1600 rpm?

23 February 2025 0 7K Report

For electrochemistry experiments involving hydrogen evolution reaction and oxygen reduction reactions.

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How to properly dilute RNA for cDNA?

Hi all, I have a question regarding proper dilutions and conversions of my RNA. According to my protocol, my cDNA kit is optimized for 1ug of RNA and calls for this amount. I would like to...

07 July 2024 9,206 4 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View

Scholarly Journals Accepting Submissions?

Could you share other peer-reviewed journals that have accepted your scholarship? I am seeking to publish a new article focused on diversity and student engagement or write a book chapter. Any...

19 June 2024 4,716 0 View

What reference management softwares are useful for systematic reviews?

Systematic review

21 March 2024 5,901 1 View

Who has research on healing attachment wounds in male-identified populations?

I am interested in looking at male attachment, including the development of attachment wounds, systemic and cultural factors. I’d like to specifically tie together the potential impact of healing...

10 February 2024 656 2 View

Is there a way to calculate a (robust) standard deviation using robust standard errors?

The robust standard errors were calculated using MLR.

03 November 2023 4,239 0 View

Cloning a 700bp insert into a 18kb plasmid?

Hello everybody, I'm having trouble cloning a 700bp insert into a 18kb plasmid. I'm using Neb Hifi assembly (Gibson assembly) but everytime I have no colonies on the plate. I tried many different...

08 August 2023 5,490 7 View

Gata-3, Eomes, Ror gamma T intracellular transcription factors?

Hi, does anyone have a good protocol for Gata-3 staining in particular. I have 0 staining, with my protocol (BD FoxP3 staining buffer) while Tbet worked well for example? thank you in advance

05 January 2023 3,699 1 View

Will different cell lines alter CT values in RT-qPCR?

Hello, I have extracted RNA from the Intestinal Enteroid Cell Line and have measured the expression of multiple genes using RT-qPCR. I tested two housekeeping genes GAPDH and ACTBR both showed...

07 December 2022 5,910 6 View

BV, BUV versus cFluor fluorochromes from cytek?

Hi, has anyone worked with these fluorochromes? Cytek cFluor antibodies are very expensive, but I am wondering whether they are better for 30+ marker panels in terms of compensation errors. Thank...

30 November 2022 1,370 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Self-Organizing Superorganisms—as envisaged by Nenad Sestan (2018)?

The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...

08 August 2024 3,118 0 View

Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

06 August 2024 5,023 1 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View