Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

HT22 purchase in Greece?

Hello everybody, I am looking for a specific cell line HT22 but I haven't found any company that could import it in Greece. Has anyone faced that problem before? Is there any alternative way that...

01 April 2019 1,917 3 View

Hello, Can anyone help me use krona tool for visualising my NGS data?

My files contain data in percentages or in counts.Does Krona tool need bioinformatic language to be used? Thanks in advance.

27 January 2019 1,758 2 View

How can we estimate how much is the sensitivity of a Chi Squared test to the number of classes?

Should we run the experiment with different number of classes and see if there is any difference?

23 August 2018 3,780 2 View

Why do I need to wash the entanol from ethanol stored samples with Tris-HCl before extracting DNA?

I worked at a laboratory and before extracting DNA, I washed of the ethanol with Tris-HCl, in order to get rid of all the ethanol before starting the extraction process, but I did not understand...

02 February 2018 9,725 1 View

Yeast fermentation medium recipe??

Can anyone please explain what is a fermentaion medium and a yeast suspension and how to make them? I have attached the paper that these two are mentioned and I have highlighted them in the...

03 January 2018 3,151 2 View

How can i dilute carvacrol (oil) ?

I have read in some papers that carvacrol can be diluted in Tween 80 (1%) and distilled water. I have tried this but carvacrol wasnt fully dissolved. After that i tried to dissolve carvacrol in...

09 August 2016 6,070 7 View

How I can get the Organizational Commitment Questionnaire?

Dear Dr. Meyer, I would like to inform you that I am a student candidate for a PhD in Cyprus University of Technology and my subject relates to job satisfaction, leadership style and commitment of...

27 March 2016 6,828 15 View

Is a standard curve required for each assay when we use an elisa kit?

Does anybody know if i can use some wells ( about 10 wells)of the TNF-a elisa kit in order to try my samples? I have not used a kit before and i wonder whether i can use some wells without running...

25 March 2016 5,274 17 View

Has anyone access to the Emotional Self-awareness questionnaire?

I am looking for a questionnaire which measures the emotional awareness, in other words if it is easy for someone to identify his feelings.So, if you don't have this specific questionnaire, but...

22 March 2016 4,095 2 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View