Are my cells contaminated with mycoplasma?

I suspect contamination with mycoplasma and fixed the cells with 4%PFA and stained them with Dapi. This is the image I got, I usually don't get any of those small rounded dapi foci outside the...

06 August 2020 6,412 1 View

Do you think improved varieties and better management practices will be the key to future increases in food production?

Agricultural experts generally agree that future increases in food production will come from three options:1) expanding the land area, 2) increasing the frequency in which the land is cropped, and...

03 July 2020 8,215 4 View

Do you think Golden Rice will alleviate hunger and malnutrition, especially in developing countries?

Golden Rice is a GMO rice plant that was modified to produce beta-carotene (provitamin A) in its grain. It was invented by the German scientists Prof. I. Potrykus (ETH) and Prof. P. Beyer (Univ...

20 June 2020 8,154 54 View

Is sustainable agriculture the same as ecological agriculture?

"Sustainable agriculture" and ecological agriculture" are two very popular terms for both government and non-government workers. Many people use them interchangeably thereby causing a lot of...

01 June 2020 1,624 100 View

What is the explanation for the "no correlation" between soil test and plant tissue test values?

In our recent research on sweetpotato here in Leyte, Philippines, we have observed a no correlation between soil test and plant tissue test values for N, P and K. Marschner (1995) in his classic...

27 April 2020 5,357 18 View

Purification of histag protein using HISTRAP HP (GE). What is the best lysis buffer and which protocol may be most suitable?

Purification of histag protein using HISTRAP HP (GE). I am working with insect cells (Tn5B) and my antigens are expressed in the nucleus. I am having difficulty lysing the cells because we have no...

10 March 2020 8,609 2 View

How to estimate the penetration thickness of light in a solid sample?

I have a solid sample and I need to determine the depth of light penetration to produce a pellet and elucidate some optical phenomenons.

27 May 2019 3,067 1 View

Where can I find information about contractual practices that stimulate performance in urban transport by bus?

I would like to learn more about the contract design of urban transport by bus that stimulate competition and better performance. Among the elements that I would like to learn more about...

02 April 2019 7,562 1 View

How can I determine the method from a .raw file?

I have raw files from a ltq-velos orbitrap but I would like to know the running method used, for instance the resolution at ms1 and ms2. (Proteomics/mass-spec)

22 August 2018 6,887 4 View

No title

15 March 2018 4,243 7 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View