09 May 2025 1 2K Report

dilution with water or with water and solvent

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Why the clear zone indicating amylase activity on agar plates dissappears after some hours?

I did qualitative assay on agar plates with starch as sole carbon source for checking amylase activity, once I added iodine the clear zone appeared arround the colonies but after some hours and...

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My recombinant enzyme activity is acting weird, does anyone have an explanation, or even know how to solve this problem?

I'm working with recombinant protein, it's a protease enzyme that we use E. coli to synthesize, and usually, it's stable for up to six months in the -80 freezer. Recently, the protein activity has...

17 April 2024 4,262 3 View

Sub-cloning not working?

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How can audiologists enhance patient-centered care in their practice?

In light of recent research findings and advancements in communication strategies for individuals with hearing loss, how can audiologists enhance patient-centered care in their practice? Are there...

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Why does performance point still in elastic range ?

I have a four-story steel structure When conducting a pushover analysis and examining the results, the structure's ductility ratio at the performance point did not exceed 1, and the equivalent...

08 November 2023 934 4 View

Cadmium sulfide nanoparticles from banana extract , what is the problem?

I'm preparing Cadmium sulfide nanoparticles using banana extract as a capping agent, after drying it, it looks like thin sheets, cannot be grinded as powder (The last time, it does not look like...

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Safe cities for women with disabilities ?

Safe cities for women with disabilities

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Can I use QIAamp DNA Stool Mini Kit for DNA extraction from a bacterial colony? if anyone tried this and what are the necessary protocol adjustments?

#DNA_extraction #QIAamp #Qiagen #Colony_DNA

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How to solve the problem if the sensor is not capable of transmitting data to base station in WSN. Is there any paper talking about this issue?

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Why enzymes couldnot migrate from wells to stacking gel in electrophoresis? Is there is problem in its precipitation?

i have precipitated enzyme by acetone.. when i use coomassie staining in native gel electrophoresis, it couldnot migrate to stacking and then resolving gel. is there is a problem in acetone...

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