How to remove PLP cofactor from SufS and IscS protein (Enzyme) to make Apo confirmation to Holo confirmation?

How to remove PLP cofactor from SufS and IscS (Enzyme) to make Apo confirmation to Holo confirmation. To confirmation that all protein in the same confirmation of the Protein. i want to remove PLP...

10 November 2019 5,146 3 View

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04 May 2018 3,062 4 View

The positive control is'nt working during enzyme activity test?

I'm working on enzyme activity of SufS for Iron-Sulfur Cluster. I have a problem of positive control, while testing my gene (Enzyme) that show no activity, the procedure is follow. Positive...

10 November 2017 5,561 4 View

Can any one give me a possible reason why my gene was not amplify by PCR, i get always dimmer instead of desire gene?

I am working of T. thermophilus bacteria and i want to amplify SufS gene, but always i just got the dimmer at the end. i change the primers but same problem, i just got dimmer, any one please?

11 December 2016 6,175 14 View

Why doesn't my DNA sample run on agarose gel?

After PCR I ran my DNA sample on agarose gel and followed the standard method provided by the company. The ladder is run but I have no DNA bends on the sample wells. But in the rt-PCR I get clear...

02 March 2014 5,552 31 View

How can we extract reducing sugar from potato tuber?

We can say that reducing sugar can be extracted from a potato 3 months after harvesting. But we have not extracted in this period. If you have any protocol for this, please share it. Please...

02 March 2014 9,469 1 View

How can I isolate the DNA from urine?

I am going to conducted a study that isolates the DNA from urine. Which method should I used to perform the experiment? I need to perform a DNA isolation method in the lab and not the prepared kit...

01 February 2014 5,494 7 View

What is the most pathogenic and resistant bacteria?

Which bacteria is the most pathogenic resistant, other then Step. aerious, worldwide? Step. aerious is well-known to be pathogenic and resistant but what is the second runner up?

01 February 2014 5,131 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View