How to remove PLP cofactor from SufS and IscS protein (Enzyme) to make Apo confirmation to Holo confirmation?

How to remove PLP cofactor from SufS and IscS (Enzyme) to make Apo confirmation to Holo confirmation. To confirmation that all protein in the same confirmation of the Protein. i want to remove PLP...

10 November 2019 5,146 3 View

No title

04 May 2018 3,062 4 View

The positive control is'nt working during enzyme activity test?

I'm working on enzyme activity of SufS for Iron-Sulfur Cluster. I have a problem of positive control, while testing my gene (Enzyme) that show no activity, the procedure is follow. Positive...

10 November 2017 5,561 4 View

Can any one give me a possible reason why my gene was not amplify by PCR, i get always dimmer instead of desire gene?

I am working of T. thermophilus bacteria and i want to amplify SufS gene, but always i just got the dimmer at the end. i change the primers but same problem, i just got dimmer, any one please?

11 December 2016 6,175 14 View

Why doesn't my DNA sample run on agarose gel?

After PCR I ran my DNA sample on agarose gel and followed the standard method provided by the company. The ladder is run but I have no DNA bends on the sample wells. But in the rt-PCR I get clear...

02 March 2014 5,552 31 View

if someone published their paper in a journal without ISI Thomson router indexed, it the article still acceptable?

Those journals that were not indexed by ISI Thomson router or indexed by other database such as Scopus, Google scholar etc. are OK for publication or not.

01 February 2014 1,454 2 View

How can I isolate the DNA from urine?

I am going to conducted a study that isolates the DNA from urine. Which method should I used to perform the experiment? I need to perform a DNA isolation method in the lab and not the prepared kit...

01 February 2014 5,494 7 View

What is the most pathogenic and resistant bacteria?

Which bacteria is the most pathogenic resistant, other then Step. aerious, worldwide? Step. aerious is well-known to be pathogenic and resistant but what is the second runner up?

01 February 2014 5,131 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View