Abdul Majid

9 Questions 12 Answers 0 Followers

Questions related from Abdul Majid

How to remove PLP cofactor from SufS and IscS protein (Enzyme) to make Apo confirmation to Holo confirmation?

How to remove PLP cofactor from SufS and IscS (Enzyme) to make Apo confirmation to Holo confirmation. To confirmation that all protein in the same confirmation of the Protein. i want to remove PLP...

11 November 2019 5,264 3 View

How can i used Na2S (1–100 μM) is for calibration in Cysteine desulfurase activity?

Reactions are carried out in 50 mM Tris–HCl, pH 7.5, 5 mM MgCl2 and 100 mM NaCl using 2 µM cysteine desulfurase (Nfs) in presence or not of 2 µM SufE1. BSA 2 µM can be used as control. In addition...

05 May 2018 3,157 4 View

The positive control is'nt working during enzyme activity test?

I'm working on enzyme activity of SufS for Iron-Sulfur Cluster. I have a problem of positive control, while testing my gene (Enzyme) that show no activity, the procedure is follow. Positive...

11 November 2017 5,679 4 View

Can any one give me a possible reason why my gene was not amplify by PCR, i get always dimmer instead of desire gene?

I am working of T. thermophilus bacteria and i want to amplify SufS gene, but always i just got the dimmer at the end. i change the primers but same problem, i just got dimmer, any one please?

12 December 2016 6,261 14 View

Why doesn't my DNA sample run on agarose gel?

After PCR I ran my DNA sample on agarose gel and followed the standard method provided by the company. The ladder is run but I have no DNA bends on the sample wells. But in the rt-PCR I get clear...

03 March 2014 5,687 31 View

How can we extract reducing sugar from potato tuber?

We can say that reducing sugar can be extracted from a potato 3 months after harvesting. But we have not extracted in this period. If you have any protocol for this, please share it. Please...

03 March 2014 9,562 1 View

if someone published their paper in a journal without ISI Thomson router indexed, it the article still acceptable?

Those journals that were not indexed by ISI Thomson router or indexed by other database such as Scopus, Google scholar etc. are OK for publication or not.

02 February 2014 1,547 2 View

How can I isolate the DNA from urine?

I am going to conducted a study that isolates the DNA from urine. Which method should I used to perform the experiment? I need to perform a DNA isolation method in the lab and not the prepared kit...

02 February 2014 5,577 7 View

What is the most pathogenic and resistant bacteria?

Which bacteria is the most pathogenic resistant, other then Step. aerious, worldwide? Step. aerious is well-known to be pathogenic and resistant but what is the second runner up?

02 February 2014 5,221 5 View