If somebody have done transwell migration assay using Huh7 cells can comment onto this?

11 February 2016 0 2K Report

I am working on to the ATP induced migration of Huh7 cells via activation of P2Y receptors.

1. I need to know whether it would be logical to use serum free media in both insert and the plate well. (0% FBS in both wells)

2. If you would to see the effect of ATP would you be treating the cells with ATP or just adding the ATP into lower chamber media (Plate well).

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