If one has a small sample and used a purposeful sample, should one be concerned with normal distribution in a t-test analysis with repeated measures?

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How to handle HIV viral RNA to isolate it properly for further sequencing?

I've been using the Qiagen Circulating extraction kit to extract the viral RNA from plasma samples. Further I used an in-house RT step (very similar to SuperScript IV from invitrogen) to get cDNA....

12 March 2024 10,022 0 View

Probe-based qPCR not reaching plateau phase?

Hi, I'm in the process of developing a hydrolysis probe qPCR assay to quantify gene copy numbers of Microcystis 16S rDNA. In troubleshooting my cycling parameters, I'm getting resulting plots such...

15 January 2024 6,957 1 View

Statistical test for optimization experiment?

Hello, I'm working on an optimization experiment related to yeast growth and the best conditions for growth. Optical density is a measure of yeast growth, and from the literature, this yeast...

01 July 2023 7,486 1 View

How do I identify content experts in the confidence in estimated treatment effects?

26 November 2022 5,477 2 View

How should I approach applying for ethics for participant observation in healthcare planning meetings?

I would like some input please. I am conducting a process evaluation of a novel community health worker intervention in England. I am involved with the steering groups as a participant observer,...

16 February 2022 9,446 6 View

How useful are surveys in case studies?

I'm conducting a comparative case study of a community health worker pilot in three sites in England. I want to identify barriers and facilitators to the implementation through interviews with...

02 January 2022 3,503 4 View

Sequence obtained from rapid cDNA ends amplification did not align with the computed sequence?

recently i have been trying to amplify a p450 gene but it kept failing. I was able to amplify a small fragment of the gene but never the whole gene so i decided to go for RACE experiment. With...

20 November 2021 7,036 3 View

Size of cDNA?

Is there a site which determines the size of cDNAs? If someone wants to insert a gene with a fluor tag such as GFP, how would they find out the size of the cDNA for that gene + GFP?

02 November 2021 5,715 3 View

Getting a pcr band larger than expected, why?

Hi, im trying to amplify the gene of size 1.4 kb, however, i ran the gel and found the band of size apx 1.6 kb instead? Can anyone please explain to me the possible reasons behind this?

07 September 2021 1,212 1 View

Can anyone recommend me books or links useful for a newbie in genetics?

Im recently working on cloning and stuck with PCR amplification and im having a hard time with snapgene and all - -the plasmid construction, the primer design Could anyone recommend me what...

07 September 2021 9,044 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View