Hi,
I want to freeze D0 pups n OCT blocks while they have been cut through the median plane to get the symmetrical images of different organs in each half of the animal body.
I just not sure if I can get a sagittal section of such a big tissue block using cryostat?
It is bad idea. Because:
1. Too bug object for section for freezing. It needs to much time
2. Too big object for cutting
3. Bones are not so good cutted in cryostat.
I reckon it is not a good idea, I am not sure about the exact size of D0 pups but I am sure they are way too big to get good quality sections from them because of the size and the heterogeneity of the different tissues. Even thought everything is going to be freezing when sectioning, the different tissues behave differently when cut; and bones, even though not fully hardened will definitely cause a problem.
Thanks for your comments. I felt that it is not the best idea. My goal is to get the dorsal ganglia in D0 pups and it's very hard to dissect them. Also to get a good orientation.
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