If half of your cells die after 3 days cytotoxic drug treatment, what best time/times you collect RNA for miRNA study?

02 September 2016 1 6K Report

I always sees cells so reach 80% at last day treatment. This is not my question. My question is of I do cell viability essay and find IC 50 at day 3, when exactly shall I do RNA collection for miRNA.

Rodrigo Andaur

To know the exactly time is very difficult, because levels of miRNAs are changing in the time. I recommend to you to collect DNA at different times.

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Science method

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