How to make sure that ultrasonic lysis of E.coli is done completely?

What's the criteria of comolete lysis of E.coli when breaking them using ultrasonic? What phenomena can help to determine if the cell are broken completely? From smell, transparence of the lysis...

07 January 2021 1,911 3 View

What is the difference between "pesticides" and "insecticides"? Are they same?

What is the difference between "pesticides" and "insecticides" in English? Are they the equivalent replacement when used in text? I am an ecological researcher who particularly concerns the...

04 January 2021 6,230 37 View

Ungrounded CPW fed slot-ring patch antenna not behaving as an antenna in CST simulation?

Greetings everyone, I am attempting to model and simulate a slot-ring patch antenna fed by ungrounded CPW in CST, although the S11 is fine, the e-field and h-field monitors shows strange...

10 October 2020 2,228 2 View

Can we perform MOX/MSTFA derivatization in Eppendorf tubes?

Hi there! Recently I am planning to work on metabolomics using GC-MS, where derivatization is a crucial step. Here, I am wondering if that possible to derivatize extract samples with both MOX and...

24 September 2020 9,695 1 View

What is the right time course when using cycloheximide to inhibit protein synthesis?

I'm going to use cycloheximide to inhibit the synthesis of a membrane protein in HEK-293T cells, but I'm not sure the right time that needed to treat cells without influencing its viability. I...

21 September 2020 4,321 2 View

How to eliminate the warning of 'position not found when parsing line' during DAMASK simulation with ABAQUS/standard?

Hi, I am trying to use the ABAQUS/standard solver to simulate the single crystal deformation behaviour with DAMASK framework. The materials is BCC ferrite with hooke elasticity and phenopowerlaw...

10 December 2019 4,667 1 View

What is the yellow/brown salt crystal during protein crystallization?

During protein crystallization screening, we encountered several types of proteins grew yellow stick crystals with a reservoir containing 0.1 M BIS-TRIS pH 6.5, 2.0 M Ammonium sulfate. However,...

09 January 2019 2,188 1 View

Does anybody know any company manufacture cyclic olefin polymers: COP (except Zeonex/Zeon)?

Want to try COP from different vendors! Is there any one in China? Thanks!

24 April 2018 8,736 2 View

What is a quick and easy screen for visualizing the presence of gold nanoparticles?

Hi all, I am trying to design a hypothetical screen to find additional genes that are responsible for C. metallidurans' ability to reduce gold ions (Au(I) and Au(III)) to solid gold nanoparticles....

12 February 2018 7,511 2 View

One virus may have two different kinds of particle?

15 February 2017 676 10 View

In the preparation of glycerol, the intermediate compound 2-chloropropan-1,3-diol is formed after addition of HOCl to allyl alcohol. How?

When HOCl is added to propene, the product is 1-chloro 2-propanol. However, when the same HOCl adds to allyl alcohol, the expected product is 3-chloropropan-1, 2-diol. But actually,...

20 October 2019 768 3 View

In the preparation of glycerol, the intermediate compound 2-chloropropan-1,3-diol is formed after addition of HOCl to allyl alcohol. How?

When HOCl is added to propene, the product is 1-chloro 2-propanol. However, when the same HOCl adds to allyl alcohol, the expected product is 3-chloropropan-1, 2-diol. But actually,...

09 October 2019 8,232 4 View

Non-specific labelling with Click chemistry reaction?

I am working on identification of a small peptide in brain tissue. We have modified the peptide with alkyne modifications for azide-based clicking. For Click reaction we have used 50 uM CuSO4,...

23 September 2019 8,052 3 View

Non-specific labelling with Click chemistry reaction?

I am working on identification of a small peptide in brain tissue. We have modified the peptide with alkyne modifications for azide-based clicking. For Click reaction we have used 50 uM CuSO4,...

08 September 2019 8,510 2 View

Why FT-IR analysis of N-acetyl-D-glucosamine is showing weak band for alkyne bonds ?

FT-IR analysis of N-acetyl-D-glucosamine (NAG) is showing weak band at 2124 cm-1. According to the reference index, 2124 cm-1 weak band represents alkyne bond. Since there is no alkyne bond in...

30 May 2019 148 3 View

Why FT-IR analysis of N-acetyl-D-glucosamine is showing weak band for alkyne bonds ?

FT-IR analysis of N-acetyl-D-glucosamine (NAG) is showing weak band at 2124 cm-1. According to the reference index, 2124 cm-1 weak band represents alkyne bond. Since there is no alkyne bond in...

04 May 2019 6,362 3 View

Are there any bioisosteres of triple bond?

Are there any bioisosteric replacements of triple bond (alkyne) which were employed for lead optimization or scaffold hopping? Thanks.

23 April 2019 2,656 4 View

What is the function of NaHSO3/H2SO4 in oxidation of alkene with KMnO4 in alkaline medium? Is it to stop the reaction or to decolorize the MnO2?

i'm oxidizing an alkene with KMnO4 in basic medium so the double bond is dihydroxylated. i'm using a procedure from a journal and it's stated that after the mixture standed for 24 h, H2SO4 is added.

03 April 2019 5,576 1 View

What side products can you expect from hydrosilylation reaction with styrenes?

Hello, I am trying to purify a reaction product of hydrosilylation with a styrene compound using Karstedt's catalyst. However, it seems to be cumbersome for the following reason. After...

03 April 2019 2,504 1 View

Do you know catecholborane reactions yielding new boron-containing compounds?

Several articles report the use of catecholborane as mediator for stereodirected synthesis. Among products are many boron-free compounds. But, when catecholborane is treated with an...

03 April 2019 9,773 2 View