I plan to do a reaction as follows, which requires dehydration condensation with concentrated sulfuric acid. Will the terminal alkyne group be affected? I am a little confused.
One of my project requirements is to measure temperature and humidity in the growing environment of plant. The plant will be grown inside a petri dish with nutrient gel. A waterproof temperature...
28 January 2021 9,415 2 View
Selfconsistent noncollinear magnetic calculations can be done in VASP. I am wondering how VASP update the spin directions at each local site. For molecular dynamics, VASP search the next...
16 January 2021 1,398 3 View
I want to ligate a linear 1.7kbp plasmid with the EcoRI sites at two sides, but when I use the quick ligase kit from NEB to test the ligation efficiency it is hard to ligate it. Is there anyone...
08 December 2020 3,442 3 View
Hello, I'm a university student who is currently doing a plant incubator project, which requires LED grow light system. One of the project objectives is to provide a wavelength-tunable light...
25 November 2020 5,508 6 View
Recently I have been involved in this problem: After expressed by adding IPTG, lysed with B-PER, and resolved with Buffer B (8M urea), incubate with Ni-NTA beads, everything looks fine. When I...
21 November 2020 7,416 3 View
I'm currently working on my academic project and it is required to perform a simulation about ASTM D575-91 (Method B) using ANSYS. I'm totally new to ANSYS, hoping someone can help me out. "The...
28 October 2020 8,764 3 View
Hi, I was doing the Abaqus explicit model for a plain woven fabric where two types of yarns(PU&PA) were set as the weft and one type of yarn(PA) was set as the warp. When the load applied to the...
10 September 2020 1,164 2 View
I am graduate from other major, and now working in computer science and technology, my roomates are mostly researching on image recognition. I want to switch to it and learn how to cooperate with...
21 August 2020 2,888 9 View
I differentiated THP-1 cells to macrophages using PMA(100ng/ml) and then used lentivirus to deliver shRNA to knockdown my target genes. Unfortunately, it was too difficult to be transfected...
16 August 2020 5,073 3 View
Dear All : I have a curious question. Among the index performance of the LQR, X' Q X and u' R u have the physical meaning. Now, my question what is the physical meaning of the cross term X' S...
02 August 2020 1,780 1 View
I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
03 March 2021 3,661 2 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...
02 March 2021 9,271 1 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...
01 March 2021 516 7 View
I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??
28 February 2021 2,153 1 View
hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...
27 February 2021 3,882 3 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...
25 February 2021 2,225 5 View
The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...
23 February 2021 2,049 3 View