I used a SSR marker to check the Xa21 gene of rice progeny which has been generated by multi-parents. Based on the gel bands. Could you please explain that the lanes with triple bands are heterozygotes from their parents? Thanks in advance!
I need to calculate the prevalence ratios to show the trend of prevalence of a drug group used by pregnant women over the years (2001-2018). I would like to use the year 2001 with age distribution...
05 January 2021 7,986 5 View
Hi, I am wondering if the immunofluorescent staining of CD31 antibodies can both mark endothelial cells and indicating their viability. In other words, if I perform immunofluorescent staining for...
23 December 2020 5,815 4 View
I'm working with Herpesvirus and Adenovirus. For Herpesvirus, I use Vero cell line to activate and check virus titer. For Adenovirus, I use Hela cell line. Recently, I could activate them, but...
16 December 2020 4,073 5 View
Hi all, I have tried to use Crispr Cas9 ( plasmid px333_cas9_mCherry) to delete some enhancers. I designed sgRNA using Chopchop website to target both sides of an enhancer to induce homologous...
13 December 2020 942 3 View
Hi folks, I wonder if anyone know how to calculate the fractional vegetation cover (FVC) from Landsat imagery? As far as I have read about that NDVI, NDVIs, and NDVIv are three required input...
07 December 2020 4,054 2 View
As of now, I am trying to explore the potential of using high/very high-resolution satellite imagery with machine learning (ML) to identify or map forest cover (e.g. rubber). As a newbie in this...
17 November 2020 7,187 3 View
I have some samples I left in sucrose for a couple of weeks after perfusion and was wondering if these samples are still salvageable? I perfused the brain, then they were fixed in 4% pfa for 24...
15 November 2020 1,728 2 View
Dear all, These are data from gel base of cDNA sample for PCR with protocol attached on the figure. Band 5 is no reverse transcriptase enzyme during doing cDNA library used random primer. With gen...
04 October 2020 8,774 4 View
Dear all, All samples are cDNA, using reverse transcriptase enzyme to make cDNA from RNA. One group is doing PCR without DMSO, and other group is doing PCR with DMSO. One is without DMSO, the...
04 October 2020 1,320 3 View
I'm working on Western Blot and was guided to use the Sample Buffer Solution (2ME-) (x4) (Fujifilm Wako). However, Beta-metacaptoethanol (BME) is said to be necessary to cut the disulfide bond in...
13 September 2020 5,533 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...
22 February 2021 9,896 3 View
Hi, any one please guide me; SSR primers are species specific or trait specific. i have few primer which was reported in articles for wheat yield can i use these similar primer for lentil yield...
20 February 2021 315 4 View