Hello
Unfortunately, fresh fruits last few days/weeks before being spoiled in the refrigerator.
That spoilage is due to air-borne mold species such as Aspergillus, Cladosporium, botrytis and Penicillium.
How can we identify mold fungal species (apart from microscope) ??
Best way to identify mold/fungi accurately is by DNA analysis which can be relatively easy to perform using Polymerase Chain Reaction (PCR) techniques. The rRNA/rDNA sequences of fungal samples are commonly amplified using PCR and sent for DNA sequencing. I normally use universal primers such as the ITS1 and ITS4 for identification of fungal samples.
If you are not familiar with PCR techniques, the general guideline is:
1. Isolation of pure fungal sample on a suitable medium
2. Extraction of genomic DNA
3. Performing PCR amplification of sample using the chosen ITS primer
4. Sending PCR products for DNA sequencing
5. DNA sequence obtain can be analysed using online softwares such as BLAST by NCBI
You can refer to this publication for more info:
Article Fungal Identification Using Molecular Tools: A Primer for th...
Hope this helps! All the best!
Thank you Mr. Walter Aaron Wong for your valuable answer.
Yes PCR-based technics are well used to identify fungal species. What about antibodies?
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