Dear researchers: I need a protocol for identification of fatty acids in ram seminal plasma.
Thank you in advance
This link may be useful to you
http://www.ncbi.nlm.nih.gov/pubmed/26707342
Dear Rajakumar Selvaraj
i hope that you are doing well
thank you in advance
hi\YOu can use of Gc (Gass chromatography) ., with GC you can easily find your specimen FA profiles.
I evaluate them in my article recently in poultry.
please dont hesitate to call me if you have any question,
Regards,
Mehdi
Thank you for your anserws
can you send me via email you protocole with all detaille [email protected]
thank you in advance
HI Dear Friend, Larbi.
I modified another methods that you can observe in attached file; summary is:
Fatty acid analyses The fatty acid prole of sperm plasma membrane was analysed
according to Rooke et al.(2001) with minor modications.
Briey, the semen sample was centrifuged for 20 min (700g;4C) to separate the spermatozoa and seminal plasma.
Resuspending in a normal saline solution, the spermatozoa were washed twice, centrifuged for 20 min (700g;4C), and
stored at 20C. Sperm lipids were extracted, using chloroform-
methanol (2 : 1 vol/vol), dissolved in a NaOH-MeOH solution
(2%), andmethylated byBF3.Using n-hexane and saturatedNaCl
solutions, methylated fatty acids were separated and analysed by
gas chromatography (GC; Unicam4600, Cambridge, UK), using
a BPX-70 column (0.25mmi.d., 30m, 0.25-mmlm thickness; J
& W Scientic, Folsom, CA, USA) and a ame ionisation
detector (Unicam 4600, UK). The GC conditions were as
follows: injector temperature, 250 C; detector temperature,
300C; carrier gas, He; split ratio, 1 : 30; temperature program,
180C for 2 min followed by an increase to 230 Cat5 C/min, which was maintained at 24 C. Peaks were identied by comparing the retention time with those of standards (Sigma Chemical Co., St Louis, MO, USA). Nonadecanoic acid (19 : 0)
served as an internal standard and fatty acid concentration was
expressed as the percentage of total fatty acids.
Dear Pr Mohammad ,
thank you for your reponse,but i hope that a receive all detaille. the volume of sample,and the volume of chloroform-
methanol (2 : 1 vol/vol),
volume of NaOH-MeOH solution (2%)
volume of BF3.Using n-hexane
saturated NaCl solutions??
i need all detaille.
thank you in advance
Dear Pr Mohammad ,
thank you for your reponse,but i hope that a receive all detaille. the volume of sample,and the volume of chloroform-
methanol (2 : 1 vol/vol),
volume of NaOH-MeOH solution (2%)
volume of BF3.Using n-hexane
saturated NaCl solutions??
i need all detaille.
thank you in advance
Identification of fatty acid in ram seminal plasma? - ResearchGate. Available from: https://www.researchgate.net/post/Identification_of_fatty_acid_in_ram_seminal_plasma [accessed Mar 31, 2016].
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