180C for 2 min followed by an increase to 230 Cat5 C/min, which was maintained at 24 C. Peaks were identied by comparing the retention time with those of standards (Sigma Chemical Co., St Louis, MO, USA). Nonadecanoic acid (19 : 0)
served as an internal standard and fatty acid concentration was
thank you for your reponse,but i hope that a receive all detaille. the volume of sample,and the volume of chloroform-
methanol (2 : 1 vol/vol),
volume of NaOH-MeOH solution (2%)
volume of BF3.Using n-hexane
saturated NaCl solutions??
i need all detaille.
thank you in advance
Identification of fatty acid in ram seminal plasma? - ResearchGate. Available from: https://www.researchgate.net/post/Identification_of_fatty_acid_in_ram_seminal_plasma [accessed Mar 31, 2016].