This link may be useful to you

http://www.ncbi.nlm.nih.gov/pubmed/26707342

Dear Rajakumar Selvaraj 

i hope that you are doing well

thank you in advance 

hi\YOu can use of Gc (Gass chromatography) ., with GC you can easily find your specimen FA profiles.

I evaluate them in my article recently in poultry.

please dont hesitate to call me if you have any question,

Regards,

Mehdi

Thank you for your anserws 

can you send me via email you protocole with all detaille [email protected]

thank you in advance 

 HI Dear Friend, Larbi.

I modified another methods that you can observe in attached file; summary is:

Fatty acid analyses The fatty acid prole of sperm plasma membrane was analysed

according to Rooke et al.(2001) with minor modications.

Briey, the semen sample was centrifuged for 20 min (700g;4C) to separate the spermatozoa and seminal plasma.

Resuspending in a normal saline solution, the spermatozoa were washed twice, centrifuged for 20 min (700g;4C), and

stored at 20C. Sperm lipids were extracted, using chloroform-

methanol (2 : 1 vol/vol), dissolved in a NaOH-MeOH solution

(2%), andmethylated byBF3.Using n-hexane and saturatedNaCl

solutions, methylated fatty acids were separated and analysed by

gas chromatography (GC; Unicam4600, Cambridge, UK), using

a BPX-70 column (0.25mmi.d., 30m, 0.25-mmlm thickness; J

& W Scientic, Folsom, CA, USA) and a ame ionisation

detector (Unicam 4600, UK). The GC conditions were as

follows: injector temperature, 250 C; detector temperature,

300C; carrier gas, He; split ratio, 1 : 30; temperature program,

180C for 2 min followed by an increase to 230 Cat5 C/min, which was maintained at 24 C. Peaks were identied by comparing the retention time with those of standards (Sigma Chemical Co., St Louis, MO, USA). Nonadecanoic acid (19 : 0)

served as an internal standard and fatty acid concentration was

expressed as the percentage of total fatty acids.

I hope it will be useful to you,

Regards,

Mehdi

Dear Pr Mohammad ,

thank you for your reponse,but i hope that a receive all detaille. the volume of sample,and the volume of  chloroform-

methanol (2 : 1 vol/vol),

volume of  NaOH-MeOH solution (2%)

volume of BF3.Using n-hexane

saturated NaCl solutions??

i need all detaille.

thank you in advance 

Dear Pr Mohammad ,

thank you for your reponse,but i hope that a receive all detaille. the volume of sample,and the volume of  chloroform-

methanol (2 : 1 vol/vol),

volume of  NaOH-MeOH solution (2%)

volume of BF3.Using n-hexane

saturated NaCl solutions??

i need all detaille.

thank you in advance 

Identification of fatty acid in ram seminal plasma? - ResearchGate. Available from: https://www.researchgate.net/post/Identification_of_fatty_acid_in_ram_seminal_plasma [accessed Mar 31, 2016].