Concentration(µl) Sample
200 85.05
400 75.68
600 64.56
800 73.92
1000 93.59
When i calculated regression coefficient with excel,
y = 0.0077x + 73.964 R² = 0.0475 IC 50 = 3112.20
but, it is totally different from the online IC50 calculator values (PDF for reference)