IC50 in a not dose-response pattern?

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Lucas de Freitas Pedrosa

Hey Maria, hope you are doing well!

How many doses did you use in your experiment?

Also, if it is a visualization problem on Prism, did you log-transform your dose values before plotting the nonlinear regression?

Maybe this link can help you further

https://www.graphpad.com/support/faqid/1726/

Best regards,

Saif Jabbar Yasir

https://www.isirv.org/site/images/stories/avg_documents/Methodology/munana_ic50_sop_for_external.pdf

https://antiviral.creative-diagnostics.com/cc50-ic50-assay.html