I have tried different procedures for yeast cell lysing and extracting lipid from it, but the yield was observed to be very meagre. Can anyone suggest me the best method for yeast lysis and extraction of intra-cellular lipids?
Thank u Dr.Kariona for ur guidance. I will try this method for sure. Could u pls tell me the proportion of chloroform and methanol, is there any change in that or shall I use the proportion as in Folch's method?