we are working on the use of selenium in improving salinity tolerance in plants. so we would like to determine selenium in plant tissues , using standard curve based on normal Se levels in plant tissues.
Hi Dr. Muayed
you can prepare stock solution by using sodium selenate solution with a known concentration and determined selenium by atomic absorption .
It would require a Hydride generator in order to have H2Se, for refs see https://books.google.it/books?hl=it&lr=&id=wLggXPmhY18C&oi=fnd&pg=PR3&dq=selenium+plant+analysis&ots=S5W9Y2LK9_&sig=p9Us0vn0frVlQqwGFSDsdyubpoE#v=onepage&q=selenium&f=false
just prepare stock solution of Na2SeO3 with a known serial concentration and determined selenium by atomic absorption then use the standard curve to calculate the unknown one
You may benefits from these two manuscripts
it depends upon your determination method and devices. Usually standard can be purchases
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