Hi G.

here is a detailed protocol, should be straightforward.

Best.

Christoph

https://www.nrel.gov/docs/fy16osti/60958.pdf

Dear, Christoph,

Thank you for your information. But the paper you attached me is a protocol for microalgal biomass. Could I used it for vegetable oil?

Dear Asanke

You can use this protocol for conversion of TAG into FAME.

For methanolysis of the fatty acids, approximately 10 mg of freeze-dried cells were weighed and placed in Teflon-stoppered glass vials, then 1 mL

chloroform and 1 mL methanol/H2SO4 (85:15 v/v) were added to

the vials, after which they were incubated at 105 C for 2 h, cooled

to room temperature, and then incubated on ice for 10 min. After

adding 0.5 mL of ice cold water the samples were thoroughly

mixed by vortexing for 1 min, and then centrifuged at 2000g. The

organic phases (bottom) were extracted by pipette and moved toclean borosilicate glass tubes, containing Na2SO4. Use the sample further to perform GC MS analysis.

You can use this method for all type of oils containing fatty acids.

You can follow this research article:

Development of semi-synthetic microbial consortia of Streptomyces coelicolor for increased production of biodiesel (fatty acid methyl esters)