I wonder whether T4 DNA ligase is capable of filling in the gap in DNA/RNA hybrid?

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Christian Q. Scheckhuber

Yes, that is possible: "T4 DNA ligase can connect blunt and cohesive ends, or repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids." (taken from the paper in the attachment)

Cheers, Christian

Article Enzymes used in molecular biology: A useful guide

Kirill Jefimov

Great! Thank you Christian!

Marc Niere

But wait, doesn´t your question refer to the polymerase activity of T4 DNA polymerase (and not the ligase activity of another enzyme from T4)? I am not sure if I understand your question, but I guess you wonder, whether T4 DNA polymerase has RNA-dependent DNA polymerase activity (i.e., reverse transcriptase activity)...? I don´t know the answer, but I don´t think so.

You are right Mark, T4 DNA ligase.