I wish to stain sections of algae (Specifically: order Ceramiales). Can anyone suggest an alternate stain for Aniline blue?

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Digvijay Singh Yadav

i wish to stain the sections of Ceramium. The fresh samples were okay as they were naturally red but those i thawed from -80o C are mostly color less and need to be stained.

Also, I am not targetting any specific molecule e.g. lipid or anything its just for visualization of cells patterns and placements.

Anthony Cheshire

Toluidine blue is great as it provides a differential colouring for acidic polysacharides. Make it up and buffer to ph 4.5