I thought it was a problem caused by the power supply so I changed it during the process, but the problem stayed the same.
Your gel is not homogenous or your stacking gel is too short to stack protein in the same line. There are to thing i suggest you to try. İncrease your stacking gel volume and load your seperating gel as sson as possible after TEMED addition.
Check the pH of the gels. Also, be sure about the pH of your samples after adding the sample buffer. The arch shape could be caused from the pH variations.
Hello! many things can be
the first and easiest to check is if you put the connection upside down (red with black-black with red) that makes them migrate upwards and that bell is formed.
the second is that your gel has irregularities, it could be small bubbles when assembling it or breakage in the glass etc.
the third is that your stacking is too small, the wells are too small for the volume of your sample or that you take too long to add the volumes seen above.
and the last one I can think of is that you have differences in concentrations and that is why some lanes run so differently.
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