which titer of viral particle should be used?
which concentration of lenti viral particles should be used?
what volume of viral particle should be used?
Hello, I'm looking to dissolve soy lecithin in order to create a liposome. I've tried using various solvents like pure ethanol, methanol, and a mixture of ethanol and methanol but they didn't...
02 August 2024 6,980 4 View
it's my first time using NVivo software.
01 August 2024 9,304 5 View
I have collect a LSV data by taking SCE as the reference electrode. But I want to convert this one to Potential with respect to Reversible Hydrogen Electrode. Please suggest me the required...
13 July 2024 7,841 2 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View
Is it a source for moisture factor in different plants? I need to find moisture factor (oven dry weight / wet weight) in plants in different studies.
10 June 2024 842 0 View
the autoclave has been smelly and seem likely yellow after my synthesis and this changes may effected on other synthesis, so I want to clean PTFE from all sulfur contamination that remain on...
31 May 2024 2,985 0 View
I have a lipopeptide solution that I intend to lyophilize in a few days. Should I put it in the -20 freezer or -80? Which temperature is best for maintaining its properties?
20 May 2024 3,893 0 View
Dear researchers, I'm currently conducting a direct cyclic fatigue simulation in Abaqus, but I'm encountering difficulties in analyzing the results. Despite the simulation running, I'm struggling...
04 May 2024 6,957 2 View
Hello. My polyacrylamide gel becomes cloudy after de staining with a solution consist of (50% miliQ water + 40% methanol + 10% acetic acid) and the background of my gel does not become completely...
02 May 2024 895 2 View
I seeded 30000 ReN progenitor cell on XF96 plate which coated with matrigel. I differentiate them for 2 weeks and read OCR with inhibitors. However nothing worked. FCCP and oligomycin were same...
17 April 2024 9,012 0 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...
11 July 2024 4,812 5 View
Get too many colonies, which are mostly non-transformants when tested by colony PCR
11 July 2024 6,775 3 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
I have searched a lot of information from the published article but couldn't find valuable. As I have prepared the Lentiviral vector and stored it in -80 degree!
21 June 2024 3,484 1 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View