Use Tris-EDTA (TE) buffer. Tris inhibits acid-catalyzed hydrolysis, while EDTA keeps it sterile and inhibits hydrolysis by enzymes, which are all metal-dependent. If you have the freedom to refrigerate, do that, and make it as cold as possible. Liquid nitrogen is very safe, and -80C too.
If you can't refrigerate, the clonesaver paper is probably your best bet. We have good experiences with TE + regular silica filter paper (GF/F). You can spot on paper and keep them in the freezer as a backup, to guard against freezer failure. Make sure everyone knows where the papers are kept, so they can grab them first when the building is burning.
Yes Tris EDTA is fine maybe EDTA 0.1mM is good enough.Im using a plasmid prep made in october 2014 even as recently till yesterday.
Ensure that the water is autoclaved and nuclease free and the prepation was made in autoclaved tips to ensure no nucleases are present
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