i think it should be between 1% and 2%?
Dear Tobias C. Wagner
Pls. find the attached files, may help in your research , good luck
http://link.springer.com/protocol/10.1385%2F1-59259-060-8%3A53
http://www.ncbi.nlm.nih.gov/pubmed/22589122
http://www.news-medical.net/life-sciences/Northern-Blot-RNA-Blot.aspx
https://www.mskcc.org/sites/default/files/node/25002/documents/Northern%20Blot%20protocol.pdf
http://www.nature.com/protocolexchange/protocols/67#/equipment
http://www.nature.com/protocolexchange/protocols/4165#/procedure
http://www.nature.com/nprot/journal/v8/n2/full/nprot.2013.006.html
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC298572/pdf/pnas00291-0091.pdf
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC308062/pdf/nar00033-0262.pdf
Regards
Prof. Houda Kawas
Yes 1 to 1.2% is fine. But use narrow lanes to run the sample with less voltage and less time like 60 V for 45 minutes to avoid RNA smearing...
i use 1% gel n run for 2h at 70V
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...
03 March 2021 2,297 3 View
03 March 2021 8,272 1 View
Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...
03 March 2021 2,037 3 View
Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...
03 March 2021 6,317 1 View
i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).
03 March 2021 7,100 1 View
03 March 2021 5,360 2 View
The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...
03 March 2021 4,107 4 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
02 March 2021 5,204 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why
02 March 2021 1,388 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View