I am a scholar and want to perform antimicrobial activity of silver nanoparticles prepared from mango plant. Do I have to perform MIC for this or antimicrobial activity can be performed directly?
In the case of testing the antimicrobial activity of silver nanoparticles prepared from a plant extract, it is recommended to perform a minimum inhibitory concentration (MIC) assay to determine the minimum concentration of the silver nanoparticles required to inhibit the growth of the microorganisms being tested. The MIC assay is a standard method for evaluating the antimicrobial activity of a substance, and is commonly used in research studies.
The MIC assay involves testing different concentrations of the silver nanoparticles against the microorganisms in question, and observing the lowest concentration at which there is no visible growth of the microorganisms after a specified incubation period. This concentration is then considered the MIC for that particular microorganism.
While it may be tempting to skip the MIC assay and perform an antimicrobial activity assay directly, it is important to note that such an approach may not provide accurate and reliable results. Without determining the MIC, it is difficult to know whether a negative result in the antimicrobial activity assay is due to the nanoparticles being ineffective or simply not being used at a high enough concentration.
So, it is recommended to perform a MIC assay to determine the minimum inhibitory concentration required to inhibit the growth of the microorganisms being tested. This will provide a reliable and accurate measure of the antimicrobial activity of the nanoparticles.
Article Green synthesis of silver nanoparticles using paper wasp‘s h...
Antibacterial activity
The procedure for testing antimicrobial activity was carried out by the method of double serial dilutions in a liquid nutrient medium - Muller-Hinton broth (MHB) at the Scientific Center of Anti-infectious Drugs (Republic of Kazakhstan, Almaty). The study of antibacterial activity using a method of double serial dilutions in broth (1) 1:1; (2) 1:2; (3) 1:4; (4)1:8; (5)1:16; (6) 1:32, (7) 1:64; (8) 1:128; (9) 1:256; (10) 1:516 (agent: broth) was conducted on the AgNPs
Article Antimicrobial and antioxidant activity of AgNPs stabilized w...